Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance.
Identifieur interne : 001874 ( Main/Exploration ); précédent : 001873; suivant : 001875Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance.
Auteurs : Samarthya Bhagia [États-Unis] ; Hongjia Li [États-Unis] ; Xiadi Gao [États-Unis] ; Rajeev Kumar [États-Unis] ; Charles E. Wyman [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2016.
Abstract
BACKGROUND
Flowthrough pretreatment is capable of removing much higher quantities of hemicellulose and lignin from lignocellulosic biomass than batch pretreatment performed at otherwise similar conditions. Comparison of these two pretreatment configurations for sugar yields and lignin removal can provide insights into lignocellulosic biomass deconstruction. Therefore, we applied liquid hot water (LHW) and extremely dilute acid (EDA, 0.05%) flowthrough and batch pretreatments of poplar at two temperatures and the same pretreatment severity for the solids. Composition of solids, sugar mass distribution with pretreatment, sugar yields, and lignin removal from pretreatment and enzymatic hydrolysis were measured.
RESULTS
Flowthrough aqueous pretreatment of poplar showed between 63 and 69% lignin removal at both 140 and 180 °C, while batch pretreatments showed about 20 to 33% lignin removal at similar conditions. Extremely dilute acid slightly enhanced lignin removal from solids with flowthrough pretreatment at both pretreatment temperatures. However, extremely dilute acid batch pretreatment did realize greater than 70% xylan yields largely in the form of monomeric xylose. Close to 100% total sugar yields were measured from LHW and EDA flowthrough pretreatments and one batch EDA pretreatment at 180 °C. The high lignin removal by flowthrough pretreatment enhanced cellulose digestibility compared to batch pretreatment, consistent with lignin being a key contributor to biomass recalcitrance. Furthermore, solids from 180 °C flowthrough pretreatment were much more digestible than solids pretreated at 140 °C despite similar lignin and extensive hemicellulose removal.
CONCLUSIONS
Results with flowthrough pretreatment show that about 65-70% of the lignin is solubilized and removed before it can react further to form low solubility lignin rich fragments that deposit on the biomass surface in batch operations and hinder enzyme action. The leftover 30-35% lignin in poplar was a key player in biomass recalcitrance to enzymatic deconstruction and it might be more difficult to dislodge from biomass with lower temperature of pretreatment. These results also point to the possibility that hemicellulose removal is more important as an indicator of lignin disruption than in playing a direct role in reducing biomass recalcitrance.
DOI: 10.1186/s13068-016-0660-5
PubMed: 27833657
PubMed Central: PMC5103384
Affiliations:
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Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Li, Hongjia" sort="Li, Hongjia" uniqKey="Li H" first="Hongjia" last="Li">Hongjia Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Gao, Xiadi" sort="Gao, Xiadi" uniqKey="Gao X" first="Xiadi" last="Gao">Xiadi Gao</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Flowthrough pretreatment is capable of removing much higher quantities of hemicellulose and lignin from lignocellulosic biomass than batch pretreatment performed at otherwise similar conditions. Comparison of these two pretreatment configurations for sugar yields and lignin removal can provide insights into lignocellulosic biomass deconstruction. Therefore, we applied liquid hot water (LHW) and extremely dilute acid (EDA, 0.05%) flowthrough and batch pretreatments of poplar at two temperatures and the same pretreatment severity for the solids. Composition of solids, sugar mass distribution with pretreatment, sugar yields, and lignin removal from pretreatment and enzymatic hydrolysis were measured.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Flowthrough aqueous pretreatment of poplar showed between 63 and 69% lignin removal at both 140 and 180 °C, while batch pretreatments showed about 20 to 33% lignin removal at similar conditions. Extremely dilute acid slightly enhanced lignin removal from solids with flowthrough pretreatment at both pretreatment temperatures. However, extremely dilute acid batch pretreatment did realize greater than 70% xylan yields largely in the form of monomeric xylose. Close to 100% total sugar yields were measured from LHW and EDA flowthrough pretreatments and one batch EDA pretreatment at 180 °C. The high lignin removal by flowthrough pretreatment enhanced cellulose digestibility compared to batch pretreatment, consistent with lignin being a key contributor to biomass recalcitrance. Furthermore, solids from 180 °C flowthrough pretreatment were much more digestible than solids pretreated at 140 °C despite similar lignin and extensive hemicellulose removal.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Results with flowthrough pretreatment show that about 65-70% of the lignin is solubilized and removed before it can react further to form low solubility lignin rich fragments that deposit on the biomass surface in batch operations and hinder enzyme action. The leftover 30-35% lignin in poplar was a key player in biomass recalcitrance to enzymatic deconstruction and it might be more difficult to dislodge from biomass with lower temperature of pretreatment. These results also point to the possibility that hemicellulose removal is more important as an indicator of lignin disruption than in playing a direct role in reducing biomass recalcitrance.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27833657</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance.</ArticleTitle><Pagination><MedlinePgn>245</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Flowthrough pretreatment is capable of removing much higher quantities of hemicellulose and lignin from lignocellulosic biomass than batch pretreatment performed at otherwise similar conditions. Comparison of these two pretreatment configurations for sugar yields and lignin removal can provide insights into lignocellulosic biomass deconstruction. Therefore, we applied liquid hot water (LHW) and extremely dilute acid (EDA, 0.05%) flowthrough and batch pretreatments of poplar at two temperatures and the same pretreatment severity for the solids. Composition of solids, sugar mass distribution with pretreatment, sugar yields, and lignin removal from pretreatment and enzymatic hydrolysis were measured.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Flowthrough aqueous pretreatment of poplar showed between 63 and 69% lignin removal at both 140 and 180 °C, while batch pretreatments showed about 20 to 33% lignin removal at similar conditions. Extremely dilute acid slightly enhanced lignin removal from solids with flowthrough pretreatment at both pretreatment temperatures. However, extremely dilute acid batch pretreatment did realize greater than 70% xylan yields largely in the form of monomeric xylose. Close to 100% total sugar yields were measured from LHW and EDA flowthrough pretreatments and one batch EDA pretreatment at 180 °C. The high lignin removal by flowthrough pretreatment enhanced cellulose digestibility compared to batch pretreatment, consistent with lignin being a key contributor to biomass recalcitrance. Furthermore, solids from 180 °C flowthrough pretreatment were much more digestible than solids pretreated at 140 °C despite similar lignin and extensive hemicellulose removal.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Results with flowthrough pretreatment show that about 65-70% of the lignin is solubilized and removed before it can react further to form low solubility lignin rich fragments that deposit on the biomass surface in batch operations and hinder enzyme action. The leftover 30-35% lignin in poplar was a key player in biomass recalcitrance to enzymatic deconstruction and it might be more difficult to dislodge from biomass with lower temperature of pretreatment. These results also point to the possibility that hemicellulose removal is more important as an indicator of lignin disruption than in playing a direct role in reducing biomass recalcitrance.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bhagia</LastName><ForeName>Samarthya</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hongjia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Xiadi</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Batch</Keyword><Keyword MajorTopicYN="N">Dilute acid</Keyword><Keyword MajorTopicYN="N">Flowthrough</Keyword><Keyword MajorTopicYN="N">Lignocellulosic biomass</Keyword><Keyword MajorTopicYN="N">Liquid hot water</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Pretreatment</Keyword><Keyword 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last="Wyman">Charles E. Wyman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Bois
|area= PoplarV1
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|clé= pubmed:27833657
|texte= Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance.
}}
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